Prince Of Penzance will not defend his Melbourne Cup title after suffering an injury at Caulfield on Saturday.

The seven-year-old could finish only fourth in the Group Two Herbert Power Stakes at the Victoria track and was found to be lame after the race.

Trainer Darren Weir was initially unsure as to the extent of the injury, but the Racing Victoria stewards later confirmed Prince Of Penzance will require surgery on a leg injury.

The RV Stewards tweeted: “Prince of Penzance – suffered injury to off fore, requires surgery. Out for the spring.”

Prince Of Penzance was famously ridden to victory in last year’s Melbourne Cup by Michelle Payne, who became the first female rider to win the race.